Slice Beef: Cut the beef into thin, even strips against the grain for tender jerky.
Mix Marinade: Combine soy sauce, Worcestershire sauce, brown sugar, apple cider vinegar, smoked paprika, garlic powder, onion powder, black pepper, and cayenne pepper in a bowl.
Marinate: Place the beef strips in the marinade, ensuring they are well coated. Refrigerate for at least 4 hours, preferably overnight.
Dehydrator Preparation: Preheat your dehydrator to 160°F (71°C).
Arrange Beef: Drain any excess marinade off the beef strips and arrange them on the dehydrator trays, leaving some space between each piece.
Dehydrate: Dehydrate the beef for 4 to 8 hours, checking periodically for your desired level of dryness.
Cool and Store: Let the jerky cool before storing it in an airtight container.